Thomas R. Ward - NCCR MSE

Thomas R. Ward

Department of Chemistry, University of Basel

Director of NCCR Molecular Systems Engineering

Project Leader

Publications

I. Casas-Rodrigo, T. Vornholt, K. Castiglione, T. M. Roberts, M. Jeschek, T. R. Ward, S. Panke “Permeabilisation of the Outer Membrane of for Enhanced Transport of Complex Molecules“ Microb. Biotechnol. 2025. [DOI]
I. Morita, A. Faraone, E. Salvisberg, K. L. Zhang, R. P. Jakob, T. Maier, T. R. Ward “Directed Evolution of an Artificial Hydroxylase Based on a Thermostable Human Carbonic Anhydrase Protein“ ACS Catal. 2024. [DOI]
G. Renno, D. Chen, Q. X. Zhang, R. M. Gomila, A. Frontera, N. Sakai, T. R. Ward, S. Matile “Pnictogen-Bonding Enzymes“ Angew. Chem. Int. Ed. 2024. [DOI]
M. Mukherjee, V. Waser, E. F. Morris, N. V. Igareta, A. H. Follmer, R. P. Jakob, D. Üzümcü, T. Maier, T. R. Ward “Artificial Peroxidase Based on the Biotin–Streptavidin Technology that Rivals the Efficiency of Natural Peroxidases“ ACS Catal. 2024. [DOI]
D. Chen, X. Zhang, A. A. Vorobieva, R. Tachibana, A. Stein, R. P. Jakob, Z. Zou, D. A. Graf, A. Li, T. Maier, B. Correia, T. R. Ward “An evolved artificial radical cyclase enables the construction of bicyclic terpenoid scaffolds via an H-atom transfer pathway“ Nat. Chem. 2024. [DOI]
K. Yu, R. Tachibana, C. Rumo, N. V. Igareta, K. L. Zhang, T. R. Ward “Artificial Metalloenzyme-Catalyzed Enantioselective Carboamination of Alkenes“ ChemCatChem 2024. [DOI]
Z. Zou, S. Wu, D. Gerngross, B. Lozhkin, D. Chen, R. Tachibana, T. R. Ward “Combining an artificial metathase with a fatty acid decarboxylase in a whole cell for cycloalkene synthesis“ Nat. Synth. 2024. [DOI]
X. Zhang, D. P. Chen, J. Stropp, R. Tachibana, Z. Zou, D. Klose, T. R. Ward “Repurposing myoglobin into an abiological asymmetric ketoreductase“ Chem-Us 2024. [DOI]
Z. Zou, B. Higginson, T. R. Ward “Creation and optimization of artificial metalloenzymes: Harnessing the power of directed evolution and beyond“ Chem-Us 2024. [DOI]
T. Vornholt, M. Mutny, G. W. Schmidt, C. Schellhaas, R. Tachibana, S. Panke, T. R. Ward, A. Krause, M. Jeschek “Enhanced Sequence-Activity Mapping and Evolution of Artificial Metalloenzymes by Active Learning“ ACS Cent. Sci. 2024. [DOI]
A. Baiyoumy, R. Vinck, T. R. Ward “The Two Janus Faces of CpRu-Based Deallylation Catalysts and Their Application for in Cellulo Prodrug Uncaging“ Helv. Chim. Acta 2024. [DOI]
K. Yu, K. Zhang, R. P. Jakob, T. Maier, T. R. Ward “An artificial nickel chlorinase based on the biotin–streptavidin technology“ Chem. Commun. 2024. [DOI]
T. Vornholt, Z. Jončev, V. Sabatino, S. Panke, T. R. Ward, C. Sparr, M. Jeschek “An Artificial Metalloenzyme for Atroposelective Metathesis**“ ChemCatChem 2023. [DOI]
K. Yu, Z. Zou, N. V. Igareta, R. Tachibana, J. Bechter, V. Köhler, D. Chen, T. R. Ward “Artificial Metalloenzyme-Catalyzed Enantioselective Amidation via Nitrene Insertion in Unactivated C(sp3)–H Bonds“ J. Am. Chem. Soc. 2023. [DOI]
N. V. Igareta, R. Tachibana, D. C. Spiess, R. L. Peterson, T. R. Ward “Spiers Memorial Lecture: Shielding the active site: a streptavidin superoxide-dismutase chimera as a host protein for asymmetric transfer hydrogenation“ Faraday Discuss. 2023. [DOI]
S. Hirschi, T. R. Ward, W. P. Meier, D. J. Müller, D. Fotiadis “Synthetic Biology: Bottom-Up Assembly of Molecular Systems“ Chem. Rev. 2022. [DOI]
J. Vallapurackal, A. Stucki, A. D. Liang, J. Klehr, P. Dittrich, T. R. Ward “Ultrahigh-Throughput Screening of an Artificial Metalloenzyme using Double Emulsions“ Angew. Chem. Int. Ed. 2022. [DOI]
M. R. Schreier, X. Guo, B. Pfund, Y. Okamoto, T. R. Ward, C. Kerzig, O. Wenger “Water-Soluble Tris(cyclometalated) Iridium(III) Complexes for Aqueous Electron and Energy Transfer Photochemistry“ Acc. Chem. Res. 2022. [DOI]
A. Stein, A. D. Liang, R. Sahin, T. R. Ward “Incorporation of metal-chelating unnatural amino acids into halotag for allylic deamination“ J. Organomet. Chem. 2022. [DOI]
C. Miró-Vinyals, A. Stein, S. Fischer, T. R. Ward, A. Deliz Liang “HaloTag Engineering for Enhanced Fluorogenicity and Kinetics with a Styrylpyridium Dye“ ChemBioChem 2021. [DOI]
A. Stein, D. Chen, N. V. Igareta, Y. Cotelle, J. G. Rebelein, T. R. Ward “A Dual Anchoring Strategy for the Directed Evolution of Improved Artificial Transfer Hydrogenases Based on Carbonic Anhydrase“ ACS Cent. Sci. 2021. [DOI]
A. Baiyoumy, J. Vallapurackal, F. Schwizer, T. Heinisch, T. Kardashliev, M. Held, S. Panke, T. R. Ward “Directed Evolution of a Surface-Displayed Artificial Allylic Deallylase Relying on a GFP Reporter Protein“ ACS Catal. 2021. [DOI]
B. Lozhkin, T. R. Ward “Bioorthogonal strategies for the in vivo synthesis or release of drugs“ Bioorg. Med. Chem. 2021. [DOI]
F. Christoffel, N. V. Igareta, M. M. Pellizzoni, L. Tiessler-Sala, B. Lozhkin, D. C. Spiess, A. Lledós, J. Maréchal, R. L. Peterson, T. R. Ward “Design and evolution of chimeric streptavidin for protein-enabled dual gold catalysis“ Nat. Catal. 2021. [DOI]
S. Di Leone, J. Vallapurackal, S. Yorulmaz Avsar, M. Kyropolou, T. R. Ward, C. G. Palivan, W. Meier “Expanding the Potential of the Solvent-Assisted Method to Create Bio-Interfaces from Amphiphilic Block Copolymers“ Biomacromolecules 2021. [DOI]
S. Fischer, T. R. Ward, A. D. Liang “Engineering a Metathesis-Catalyzing Artificial Metalloenzyme Based on Halotag“ ACS Catal. 2021. [DOI]
A. Stucki, J. Vallapurackal, T. R. Ward, P. Dittrich “Droplet Microfluidics and Directed Evolution of Enzymes: an Intertwined Journey“ Angew. Chem. Int. Ed. 2021. [DOI]
T. Vornholt, F. Christoffel, M. M. Pellizzoni, S. Panke, T. R. Ward, M. Jeschek “Systematic Engineering of Artificial Metalloenzymes for New-to-Nature Reactions“ Sci. Adv. 2021. [DOI]
A. Samanta, V. Sabatino, T. R. Ward, A. Walther “Functional and morphological adaptation in DNA protocells via signal processing prompted by artificial metalloenzymes“ Nat. Nanotechnol. 2020. [DOI]
H. J. Davis, D. Häussinger, T. R. Ward, Y. Okamoto “A visible-light promoted amine oxidation catalyzed by a Cp*Ir complex“ ChemCatChem 2020. [DOI]
V. Sabatino, D. Staub, T. R. Ward “Synthesis of N-Substituted Indoles via Aqueous Ring-Closing Metathesis“ Catal. Lett. 2020. [DOI]
J. Serrano-Plana, C. Rumo, J. G. Rebelein, R. L. Peterson, M. Barnet, T. R. Ward “Enantioselective Hydroxylation of Benzylic C(sp3)–H Bonds by an Artificial Iron Hydroxylase Based on the Biotin–Streptavidin Technology“ J. Am. Chem. Soc. 2020. [DOI]
X. Guo, Y. Okamoto, M. R. Schreier, T. R. Ward, O. Wenger “Reductive Amination and Enantioselective Amine Synthesis by Photoredox Catalysis“ Eur. J. Org. Chem. 2020. [DOI]
S. Wu, Y. Zhou, D. Gerngross, M. Jeschek, T. R. Ward “Chemo-enzymatic cascades to produce cycloalkenes from bio-based resources“ Nat. Commun. 2019. [DOI]
S. Wu, Y. Zhou, J. G. Rebelein, M. Kuhn, H. Mallin, J. Zhao, N. V. Igareta, T. R. Ward “Breaking Symmetry: Engineering Single-Chain Dimeric Streptavidin as Host for Artificial Metalloenzymes“ J. Am. Chem. Soc. 2019. [DOI]
V. Sabatino, J. G. Rebelein, T. R. Ward ““Close-to-Release”: Spontaneous Bioorthogonal Uncaging Resulting from Ring-Closing Metathesis“ J. Am. Chem. Soc. 2019. [DOI]
H. J. Davis, T. R. Ward “Artificial Metalloenzymes: Challenges and Opportunities“ ACS Cent. Sci. 2019, 5(7):1120-36. [DOI]
Y. Cheng, L. Zong, J. López-Andarias, E. Bartolami, Y. Okamoto, T. R. Ward, N. Sakai, S. Matile “Cell-Penetrating Dynamic-Covalent Benzopolysulfane Networks“ Angew. Chem. Int. Ed. 2019. [DOI]
J. G. Rebelein, Y. Cotelle, B. Garabedian, T. R. Ward “Chemical Optimization of Whole-Cell Transfer Hydrogenation Using Carbonic Anhydrase as Host Protein“ ACS Catal. 2019. [DOI]
A. D. Liang, J. Serrano-Plana, R. L. Peterson, T. R. Ward “Artificial Metalloenzymes Based on the Biotin–Streptavidin Technology: Enzymatic Cascades and Directed Evolution“ Acc. Chem. Res. 2019. [DOI]
M. Szponarski, F. Schwizer, T. R. Ward, K. Gademann “On-cell catalysis by surface engineering of live cells with an artificial metalloenzyme“ Commun. Chem. 2018. [DOI]
J. Zhao, J. G. Rebelein, H. Mallin, C. Trindler, M. M. Pellizzoni, T. R. Ward “Genetic Engineering of an Artificial Metalloenzyme for Transfer Hydrogenation of a Self-Immolative Substrate in Escherichia coli’s Periplasm“ J. Am. Chem. Soc. 2018. [DOI]
M. Hestericová, T. Heinisch, M. Lenz, T. R. Ward “Ferritin Encapsulation of Artificial Metalloenzymes: Engineering a Tertiary Coordination Sphere for an Artificial Transfer Hydrogenase“ Dalton Trans. 2018. [DOI]
X. Guo, Y. Okamoto, M. R. Schreier, T. R. Ward, O. Wenger “Enantioselective synthesis of amines by combining photoredox and enzymatic catalysis in a cyclic reaction network“ Chem. Sci. 2018. [DOI]
Y. Okamoto, R. Kojima, F. Schwizer, E. Bartolami, T. Heinisch, S. Matile, M. Fussenegger, T. R. Ward “A cell-penetrating artificial metalloenzyme regulates a gene switch in a designer mammalian cell“ Nat. Commun. 2018. [DOI]
J. Zhao, D. G. Bachmann, M. Lenz, D. G. Gillingham, T. R. Ward “An artificial metalloenzyme for carbene transfer based on a biotinylated dirhodium anchored within streptavidin“ Catal. Sci. Technol. 2018. [DOI]
S. G. Keller, B. Probst, T. Heinisch, R. Alberto, T. R. Ward “Photo‐Driven Hydrogen Evolution by an Artificial Hydrogenase Utilizing the Biotin‐Streptavidin Technology“ Helv. Chim. Acta 2018. [DOI]
H. Mallin, T. R. Ward “Streptavidin‐Enzyme Linked Aggregates for the One‐Step Assembly and Purification of Enzyme Cascades“ ChemCatChem 2018. [DOI]
J. G. Rebelein, T. R. Ward “In vivo catalyzed new-to-nature reactions“ Curr. Opin. Biotechnol. 2018. [DOI]
M. M. Pellizzoni, F. Schwizer, C. W. Wood, V. Sabatino, Y. Cotelle, S. Matile, D. N. Woolfson, T. R. Ward “Chimeric Streptavidins as Host Proteins for Artificial Metalloenzymes“ ACS Catal. 2018. [DOI]
T. Heinisch, F. Schwizer, B. Garabedian, E. Csibra, M. Jeschek, J. Vallapurackal, V. B. Pinheiro, P. Marlière, S. Panke, T. R. Ward “E. coli surface display of streptavidin for directed evolution of an allylic deallylase“ Chem. Sci. 2018, 9(24):5383-5388. [DOI]
M. Jeschek, S. Panke, T. R. Ward “Artificial Metalloenzymes on the Verge of New-to-Nature Metabolism“ Trends Biotechnol. 2017. [DOI]
S. G. Keller, A. Pannwitz, H. Mallin, O. Wenger, T. R. Ward “Streptavidin as a Scaffold for Light-Induced Long-Lived Charge Separation“ Chem. Eur. J. 2017. [DOI]
F. Schwizer, Y. Okamoto, T. Heinisch, Y. Gu, M. M. Pellizzoni, V. Lebrun, R. Reuter, V. Köhler, J. C. Lewis, T. R. Ward “Artificial Metalloenzymes: Reaction Scope and Optimization Strategies“ Chem. Rev. 2017. [DOI]
Y. Okamoto, T. R. Ward “Cross-Regulation of an Artificial Metalloenzyme“ Angew. Chem. Int. Ed. 2017. [DOI]
L. Liu, Y. Cotelle, J. Klehr, N. Sakai, T. R. Ward, S. Matile “Anion-π catalysis: bicyclic products with four contiguous stereogenic centers from otherwise elusive diastereospecific domino reactions on π-acidic surfaces“ Chem. Sci. 2017, 8:3770-74. [DOI]
M. Jeschek, M. O. Bahls, V. Schneider, P. Marlière, T. R. Ward, S. Panke “Biotin-independent Strains of Escherichia coli for Enhanced Streptavidin Production“ Metab. Eng. 2017, 40:33-40. [DOI]
Z. Liu, V. Lebrun, T. Kitanosono, H. Mallin, V. Köhler, D. Häussinger, D. Hilvert, S. Kobayashi, T. R. Ward “Upregulation of an Artificial Zymogen by Proteolysis“ Angew. Chem. Int. Ed. 2016, 55:11587-90. [DOI]
M. Jeschek, R. Reuter, T. Heinisch, C. Trindler, J. Klehr, S. Panke, T. R. Ward “Directed evolution of artificial metalloenzymes for in vivo metathesis“ Nature 2016, doi:10.1038/nature19114. [DOI] [More Information]
M. Hestericová, M. R. Correro, M. Lenz, P. F. Corvini, P. Shahgaldian, T. R. Ward “Immobilization of an artificial imine reductase within silica nanoparticles improves its performance“ Chem. Commun. 2016, 52:9462-65. [DOI]
S. G. Keller, A. Pannwitz, F. Schwizer, J. Klehr, O. Wenger, T. R. Ward “Light-driven electron injection from a biotinylated triarylamine donor to [Ru (diimine) 3] 2+-labeled streptavidin“ Org. Biomol. Chem. 2016, 14:7197-201. [DOI]
Y. Okamoto, T. R. Ward, O. Wenger “From Photodriven Charge Accumulation to Fueling Enzyme Cascades in Molecular Factories“ Chimia 2016, 6:395. [DOI]
Y. Cotelle, N. Chuard, S. Lascano, V. Lebrun, R. Wehlauch, N. Bohni, S. Lörcher, V. Postupalenko, S. Reddy, W. Meier, C. G. Palivan, K. Gademann, T. R. Ward, S. Matile “Interfacing Functional Systems“ Chimia 2016, 6:418. [DOI]
P. Rottmann, T. R. Ward, S. Panke “Compartmentalization – A Prerequisite for Maintaining and Changing an Identity“ Chimia 2016, 6:428
M. Jeschek, S. Panke, T. R. Ward “Chapter Twenty-Three-Periplasmic Screening for Artificial Metalloenzymes“ Methods Enzymol. 2016, 580:539-56. [DOI]
E. A. Miłopolska, M. Kuss-Petermann, M. Neuburger, O. Wenger, T. R. Ward “N-Heterocyclic carbene ligands bearing a naphthoquinone appendage: Synthesis and coordination chemistry“ Polyhedron 2016, 103:261-66. [DOI]
Y. Cotelle, V. Lebrun, N. Sakai, T. R. Ward, S. Matile “Anion‑π Enzymes“ ACS Cent. Sci. 2016:DOI: 10.1021/acscentsci.6b00097. [DOI] [More Information]
Y. Okamoto, V. Köhler, C. E. Paul, F. Hollmann, T. R. Ward “Efficient In Situ Regeneration of NADH Mimics by an Artificial Metalloenzyme“ ACS Catal. 2016, 6:3553. [DOI]
Y. Okamoto, V. Köhler, T. R. Ward “An NAD(P)H-Dependent Artificial Transfer Hydrogenase for Multienzymatic Cascades“ J. Am. Chem. Soc. 2016, 138:5781. [DOI]
H. Mallin, M. Hestericová, R. Reuter, T. R. Ward “Library design and screening protocol for artificial metalloenzymes based on the biotin-streptavidin technology“ Nat. Protoc. 2016, 11:835. [DOI]
Y. Cotelle, S. Benz, A. Avestro, T. R. Ward, N. Sakai, S. Matile “Anion-π Catalysis of Enolate Chemistry: Rigidified Leonard Turns as a General Motif to Run Reactions on Aromatic Surfaces“ Angew. Chem. Int. Ed. 2016, 55:4275. [DOI]
T. Heinisch, M. Pellizzoni, M. Dürrenberger, C. E. Tinberg, V. Köhler, J. Klehr, D. Häussinger, D. Baker, T. R. Ward “Improving the Catalytic Performance of an Artificial Metalloenzyme by Computational Design“ J. Am. Chem. Soc. 2016, 137:10414. [DOI]
A. Chatterjee, H. Mallin, J. Klehr, J. Vallapurackal, A. D. Finke, L. Vera, M. Marsh, T. R. Ward “An enantioselective artificial Suzukiase based on the biotin–streptavidin technology “ Chem. Sci. 2016, 7:673. [DOI]
R. Reuter, T. R. Ward “Profluorescent substrates for the screening of olefin metathesis catalysts“ Beilstein J. Org. Chem. 2015, 11:1886-92. [DOI]
T. Heinisch, T. R. Ward “Latest Developments in Metalloenzyme Design and Repurposing“ Eur. J. Inorg. Chem. 2015, 2015:3406-18. [DOI]
J. Zhao, A. Kajetanowicz, T. R. Ward “Carbonic anhydrase II as host protein for the creation of a biocompatible artificial metathesase“ Org. Biomol. Chem. 2015, 13:5652-55. [DOI]
N. Fujieda, J. Schätti, E. Stuttfeld, K. Ohkubo, T. Maier, S. Fukuzumi, T. R. Ward “Enzyme repurposing of a hydrolase as an emergent peroxidase upon metal binding“ Chem. Sci. 2015, 6:4060. [DOI]
T. Quinto, D. Haussinger, V. Kohler, T. R. Ward “Artificial metalloenzymes for the diastereoselective reduction of NAD(+) to NAD(2)H“ Org. Biomol. Chem. 2015, 13:357-60. [DOI]