Martin Fussenegger - NCCR MSE

Martin Fussenegger

Department of Biosystems Science and Engineering (D-BSSE), ETH Zurich

Work Package Leader WP3: From Cellular Systems to Health Control

Project Leader


A. Bertschi, B. Stefanov, S. Xue, G. Charpin-El Hamri, A. P. Teixeira, M. Fussenegger “Controlling therapeutic protein expression via inhalation of a butter flavor molecule“ Nucleic Acids Res. 2023. [DOI]
M. Mahameed, P. Wang, S. Xue, M. Fussenegger “Engineering receptors in the secretory pathway for orthogonal signalling control“ Nat. Commun. 2022. [DOI]
S. Galvan, O. Madderson, S. Xue, A. P. Teixeira, M. Fussenegger “Regulation of Transgene Expression by the Natural Sweetener Xylose“ Adv. Sci. 2022. [DOI]
M. Mansouri, P. G. Ray, N. Franko, S. Xue, M. Fussenegger “Design of programmable post-translational switch control platform for on-demand protein secretion in mammalian cells“ Nucleic Acids Res. 2022. [DOI]
B. A. Stefanov, M. Fussenegger “Biomarker-driven feedback control of synthetic biology systems for next-generation personalized medicine“ Front. Bioeng. Biotechnol. 2022. [DOI]
B. Stefanov, M. Mansouri, G. Charpin-El Hamri, M. Fussenegger “Sunlight-Controllable Biopharmaceutical Production for Remote Emergency Supply of Directly Injectable Therapeutic Proteins“ Small 2022. [DOI]
T. Strittmatter, Y. Wang, A. Bertschi, L. Scheller, P. C. Freitag, P. G. Ray, P. Stuecheli, J. V. Schaefer, T. Reinberg, D. Tsakiris, A. Plückthun, H. Ye, M. Fussenegger “Programmable DARPin-based receptors for the detection of thrombotic markers“ Nat. Chem. Biol. 2022. [DOI]
V. Haellman, M. Pirkl, A. Akmammedov, P. Saxena, N. Beerenwinkel, R. Paro, A. P. Teixeira, M. Fussenegger “dCas9-mediated dysregulation of gene expression in human induced pluripotent stem cells during primitive streak differentiation“ Metab. Eng. 2022 [More Information]
M. Fussenegger “Autonomous push button-controlled rapid insulin release from a piezoelectrically activated subcutaneous cell implant“ Sci. Adv. 2022. [DOI]
D. Monserrat Lopez, V. Grimaudo, G. Prone, A. Flisch, A. Riedo, R. Zboray, T. Lüthi, M. MayorM. Fussenegger, P. Broekmann, P. Wurz, E. Lörtscher “Automated, 3-D and Sub-Micron Accurate Ablation-Volume Determination by Inverse Molding and X-Ray Computed Tomography“ Adv. Sci. 2022. [DOI]
M. Mansouri, M. Fussenegger “Electrogenetics: Bridging synthetic biology and electronics to remotely control the behavior of mammalian designer cells“ Current Opinion in Chemical Biology 2022. [DOI]
M. Mahameed, M. Fussenegger “Engineering Autonomous Closed-loop Designer Cells for Disease Therapy“ iScience 2022. [DOI]
M. Mahameed, S. Xue, B. Stefanov, G. C. Hamri, M. Fussenegger “Engineering a Rapid Insulin Release System Controlled By Oral Drug Administration“ Adv. Sci. 2022. [DOI]
H. Wang, M. Xie, G. Rizzi, X. Li, K. Tan, M. Fussenegger “Identification of Sclareol As a Natural Neuroprotective Cav1.3-Antagonist Using Synthetic Parkinson-Mimetic Gene Circuits and Computer-Aided Drug Discovery“ Adv. Sci. 2022. [DOI]
A. P. Teixeira, P. Stücheli, D. Ausländer, P. Schönenberger, S. Hürlemann, M. Fussenegger “CelloSelect – A synthetic cellobiose metabolic pathway for selection of stable transgenic CHO cell lines“ Metab. Eng. 2022. [DOI]
M. Mansouri, M. Fussenegger “Remote Control of Mammalian Therapeutic Designer Cells“ Cell Engineering 2021. [DOI]
N. Franko, A. P. Teixeira, S. Xue, G. Charpin-El Hamri, M. Fussenegger “Design of modular autoproteolytic gene switches responsive to anti-coronavirus drug candidates“ Nat. Commun. 2021. [DOI]
M. Mansouri, M. Fussenegger “Synthetic Biology-based Optogenetic Approaches to Control Therapeutic Designer Cells“ Curr. Opin. Syst. Biol. 2021. [DOI]
M. Mansouri, M. Fussenegger “Therapeutic cell engineering: designing programmable synthetic genetic circuits in mammalian cells“ Protein Cell 2021. [DOI]
H. V. Humeniuk, A. Gini, X. Hao, F. Coelho, N. Sakai, S. Matile, M. Fussenegger “Pnictogen-Bonding Catalysis and Transport Combined: Polyether Transporters Made In Situ“ JACS Au 2021. [DOI]
T. Strittmatter, P. Argast, P. Buchman, K. Krawczyk, M. Fussenegger “Control of gene expression in engineered mammalian cells with a programmable shear-stress inducer“ Biotechnol. Bioeng. 2021. [DOI]
B. Stefanov, A. P. Teixeira, M. Mansouri, A. Bertschi, K. Krawczyk, G. C. Hamri, S. Xue, M. Fussenegger “Genetically Encoded Protein Thermometer Enables Precise Electrothermal Control of Transgene Expression“ Adv. Sci. 2021. [DOI]
M. Mansouri, S. Xue, M. Hussherr, T. Strittmatter, G. Camenisch, M. Fussenegger “Smartphone-Flashlight-Mediated Remote Control of Rapid Insulin Secretion Restores Glucose Homeostasis in Experimental Type-1 Diabetes“ Small 2021. [DOI]
O. Madderson, A. P. Teixeira, M. Fussenegger “Emerging mammalian gene switches for controlling implantable cell therapies“ Curr. Opin. Chem. Biol. 2021. [DOI]
M. Mansouri, M. Hussherr, T. Strittmatter, P. Buchmann, S. Xue, G. Camenisch, M. Fussenegger “Smart-watch-programmed green-light-operated percutaneous control of therapeutic transgenes“ Nat. Commun. 2021. [DOI]
V. Haellman, T. Strittmatter, A. Bertschi, P. Stücheli, M. Fussenegger “A Versatile Plasmid Architecture for Mammalian Synthetic Biology (Vamsyb)“ Metab. Eng. 2021. [DOI]
F. Sedlmayer, A. Woischnig, V. Unterreiner, F. Fuchs, D. Baeschlin, N. Khanna, M. Fussenegger “5-Fluorouracil Blocks Quorum-Sensing of Biofilm-Embedded Methicillin-Resistant Staphylococcus Aureus in Mice“ Nucleic Acids Res. 2021. [DOI]
V. Haellman, P. Saxena, Y. Jiang, M. Fussenegger “Rational Design and Optimization of Synthetic Gene Switches for Controlling Cell-Fate Decisions in Pluripotent Stem Cells“ Metab. Eng. 2021. [DOI]
T. Strittmatter, S. Egli, A. Bertschi, R. Plieninger, D. Bojar, M. Xie, M. Fussenegger “Gene switch for l-glucose-induced biopharmaceutical production in mammalian cells“ Biotechnol. Bioeng. 2021. [DOI]
C. Yang, F. Sesterhenn, J. Bonet, E. A. van Aalen, L. Scheller, L. A. Abriata, J. T. Cramer, X. Wen, S. Rosset, S. Georgeon, T. Jardetzky, T. Krey, M. Fussenegger, M. Merkx, B. Correia “Bottom-up de novo design of functional proteins with complex structural features“ Nat. Chem. Biol. 2021. [DOI]
F. Teng, M. Fussenegger “Shedding Light on Extracellular Vesicle Biogenesis and Bioengineering“ Adv. Sci. 2020. [DOI]
M. Xie, M. Viviani, M. Fussenegger “Engineering Precision Therapies: Lessons and Motivations from the Clinic“ Synth. Biol. 2020. [DOI]
D. Ausländer, P. F. Lang, M. Kemi, M. Fussenegger “Design of Multipartite Transcription Factors for Multiplexed Logic Genome Integration Control in Mammalian Cells“ ACS Synth. Biol. 2020. [DOI]
M. Mansouri, S. Lichtenstein, T. Strittmatter, P. Buchmann, M. Fussenegger “Construction of a Multiwell Light-Induction Platform for Traceless Control of Gene ExpressionGeneexpression in Mammalian Cells“ Photoswitching Proteins : Methods and Protocols 2020. [DOI]
L. Scheller, M. Schmollack, A. Bertschi, M. Mansouri, P. Saxena, M. Fussenegger “Phosphoregulated orthogonal signal transduction in mammalian cells“ Nat. Commun. 2020. [DOI]
K. Krawczyk, S. Xue, P. Buchmann, G. Charpin-El-Hamri, P. Saxena, M. Hussherr, J. Shao, H. Ye, M. Xie, M. Fussenegger “Electrogenetic cellular insulin release for real-time glycemic control in type 1 diabetic mice“ Science 2020. [DOI]
P. Stücheli, S. Sieber, D. W. Fuchs, L. Scheller, T. Strittmatter, P. Saxena, K. Gademann, M. Fussenegger “Genetically encoded betaxanthin-based small-molecular fluorescent reporter for mammalian cells“ Nucleic Acids Res. 2020. [DOI]
C. E. Meyer, J. Liu, I. Craciun, D. Wu, H. Wang, M. Xie, M. FusseneggerC. G. Palivan “Segregated Nanocompartments Containing Therapeutic Enzymes and Imaging Compounds within DNA-Zipped Polymersome Clusters for Advanced Nanotheranostic Platform“ Small 2020. [DOI]
K. Krawczyk, L. Scheller, H. Kim, M. Fussenegger “Rewiring of endogenous signaling pathways to genomic targets for therapeutic cell reprogramming“ Nat. Commun. 2020. [DOI]
B. M. Gaub, K. C. Kasuba, E. Mace, T. Strittmatter, P. R. Laskowski, S. A. Geissler, A. Hierlemann, M. FusseneggerB. RoskaD. J. Müller “Neurons differentiate magnitude and location of mechanical stimuli“ Proc. Natl. Acad. Sci. U.S.A. 2019. [DOI]
D. Bojar, M. Fussenegger “The Role of Protein Engineering in Biomedical Applications of Mammalian Synthetic Biology“ Small 2019. [DOI]
P. Bai, Y. Liu, S. Xue, G. C. Hamri, P. Saxena, H. Ye, M. Xie, M. Fussenegger “A fully human transgene switch to regulate therapeutic protein production by cooling sensation“ Nat. Med. 2019. [DOI]
H. Chassin, M. Müller, M. Tigges, L. Scheller, M. Lang, M. Fussenegger “A modular degron library for synthetic circuits in mammalian cells“ Nat. Commun. 2019. [DOI]
L. Scheller, M. Fussenegger “From synthetic biology to human therapy: engineered mammalian cells“ Curr. Opin. Biotechnol. 2019, 58:108-16. [DOI]
H. Kim, D. Bojar, M. Fussenegger “A CRISPR/Cas9-based central processing unit to program complex logic computation in human cells“ Proc. Natl. Acad. Sci. U.S.A. 2019. [DOI]
F. Tolle, P. Stücheli, M. Fussenegger “Genetic circuitry for personalized human cell therapy“ Curr. Opin. Biotechnol. 2019, 59:31-38. [DOI]
D. Bojar, T. Fuhrer, M. Fussenegger “Purity by design: Reducing impurities in bioproduction by stimulus-controlled global translational downregulation of non-product proteins“ Metab. Eng. 2018. [DOI]
M. Mansouri, T. Strittmatter, M. Fussenegger “Light-Controlled Mammalian Cells and Their Therapeutic Applications in Synthetic Biology“ Adv. Sci. 2018. [DOI]
Y. Liu, G. Charpin-El Hamri, H. Ye, M. Fussenegger “A synthetic free fatty acid-regulated transgene switch in mammalian cells and mice“ Nucleic Acids Res. 2018. [DOI]
A. P. Teixeira, M. Fussenegger “Engineering mammalian cells for disease diagnosis and treatment“ Curr. Opin. Biotechnol. 2018. [DOI]
D. Bojar, M. Fussenegger “Programming mammalian gene expression with the antibiotic simocyclinone D8 and the flavonoid luteolin“ AIChE J. 2018. [DOI]
Y. Liu, P. Bai, A. Woischnig, G. Charpin-El Hamri, H. Ye, M. Folcher, M. Xie, N. Khanna, M. Fussenegger “Immunomimetic Designer Cells Protect Mice from MRSA Infection“ Cell 2018. [DOI]
D. Bojar, L. Scheller, G. C. Hamri, M. Xie, M. Fussenegger “Caffeine-inducible gene switches controlling experimental diabetes“ Nat. Commun. 2018. [DOI]
M. Xie, M. Fussenegger “Designing cell function: assembly of synthetic gene circuits for cell biology applications“ Nat. Rev. Mol. Cell Biol. 2018. [DOI]
Y. Okamoto, R. Kojima, F. Schwizer, E. Bartolami, T. Heinisch, S. MatileM. FusseneggerT. R. Ward “A cell-penetrating artificial metalloenzyme regulates a gene switch in a designer mammalian cell“ Nat. Commun. 2018. [DOI]
R. Kojima, M. Fussenegger “Engineering Whole Mammalian Cells for Target‐Cell‐Specific Invasion/Fusion“ Adv. Sci. 2018. [DOI]
L. Scheller, T. Strittmatter, D. Fuchs, D. Bojar, M. Fussenegger “Generalized extracellular molecule sensor platform for programming cellular behavior“ Nat. Chem. Biol. 2018. [DOI]
A. Tastanova, M. Folcher, M. Müller, G. Camenisch, A. Ponti, T. Horn, M. S. Tikhomirova, M. Fussenegger “Synthetic biology-based cellular biomedical tattoo for detection of hypercalcemia associated with cancer“ Sci. Transl. Med. 2018. [DOI]
R. Kojima, D. Bojar, G. Rizzi, G. C. Hamri, M. D. El-Baba, P. Saxena, S. Ausländer, K. R. Tan, M. Fussenegger “Designer exosomes produced by implanted cells intracerebrally deliver therapeutic cargo for Parkinson’s disease treatment“ Nat. Commun. 2018. [DOI]
H. Wang, M. Xie, G. Charpin-El Hamri, H. Ye, M. Fussenegger “Treatment of chronic pain by designer cells controlled by spearmint aromatherapy“ Nat. Biomed. Eng. 2018. [DOI]
S. Ausländer, X. Pierrat, L. Hellmann, L. Rachid, M. Fussenegger “Programmable full-adder computations in communicating three-dimensional cell cultures“ Nat. Methods 2017. [DOI]
R. Kojima, L. Scheller, M. Fussenegger “Nonimmune cells equipped with T-cell-receptor-like signaling for cancer cell ablation“ Nat. Chem. Biol. 2017. [DOI]
H. Chassin, B. Geering, L. Schukur, D. Ausländer, B. Lang, M. Fussenegger “Sensing and responding to allergic response cytokines through a genetically encoded circuit“ Nat. Commun. 2017. [DOI]
V. Haellman, M. Fussenegger “Synthetic biology – Engineering cell-based biomedical devices“ Curr. Opin. Biomed. Eng. 2017. [DOI]
F. Sedlmayer, T. Jaeger, U. Jenal, M. Fussenegger “Quorum-Quenching Human Designer Cells for Closed-Loop Control of Pseudomonas aeruginosa Biofilms“ Nano Lett. 2017. [DOI]
P. Saxena, D. Bojar, H. Zulewski, M. Fussenegger “Generation of glucose-sensitive insulin-secreting beta-like cells from human embryonic stem cells by incorporating a synthetic lineage-control network“ J. Biotechnol. 2017. [DOI]
M. Xie, D. Aubel, M. Fussenegger “Closed-loop control systems – The quest for precision therapies for diabetes“ Curr. Opin. Syst. Biol. 2017. [DOI]
A. P. Teixeira, M. Fussenegger “Synthetic biology-inspired therapies for metabolic diseases“ Curr. Opin. Biotechnol. 2017. [DOI]
D. Ausländer, M. Fussenegger “Synthetic Biology‐The Synthesis of Biology“ Angew. Chem. Int. Ed. 2017, 56:6396-419. [DOI]
S. Ausländer, M. Fussenegger “Synthetic RNA-based switches for mammalian gene expression control“ Curr. Opin. Biotechnol. 2017, 48:54-60. [DOI]
M. Muller, D. Auslander, A. Spinnler, J. Sikorski, M. Folcher, M. Fussenegger “Designed cell consortia as fragrance-programmable analog-to-digital converters“ 2017, 13:309-16. [DOI]
M. Xie, H. Ye, H. Wang, G. Charpin-El Hamri, C. Lormeau, P. Saxena, J. StellingM. Fussenegger “β-cell–mimetic designer cells provide closed-loop glycemic control“ Science 2016, 354:1296-1301. [DOI]
R. Kojima, D. Aubel, M. Fussenegger “Toward a world of theranostic medication: Programming biological sentinel systems for therapeutic intervention“ Adv. Drug Delivery Rev. 2016, 105:66-76. [DOI]
M. Xie, V. Haellman, M. FusseneggerV. Hällman “Synthetic biology-application-oriented cell engineering.“ Curr. Opin. Biotechnol. 2016, 40:139. [DOI]
P. Saxena, B. C. Heng, P. Bai, M. Folcher, H. Zulewski, M. Fussenegger “A programmable synthetic lineage-control network that differentiates human IPSCs into glucose-sensitive insulin-secreting beta-like cells“ Nat. Commun. 2016, 7:DOI:10.1038/ncomms11247. [DOI] [More Information]
P. Bai, H. Ye, M. Xie, P. Saxena, H. Zulewski, G. Charpin-El Hamri, V. Djonov, M. Fussenegger “A synthetic biology-based device prevents liver injury in mice“ J. Hepatol. 2016, 65:84. [DOI]
D. Auslaender, D. W. Fuchs, S. Huerlemann, M. Fussenegger “Engineering a ribozyme cleavage-induced split fluorescent aptamer complementation assay“ Nucleic Acids Res. 2016, 44:e94. [DOI]
A. Tastanova, A. Schulz, A. Tolstrup, A. Puklowski, H. Kaufmann, M. Fussenegger “Overexpression of YY1 increases the protein production in mammalian cells“ J. Biotechnol. 2016, 219:72. [DOI]
P. Saxena, G. Charpin-El Hamri, M. Folcher, H. Zulewski, M. Fussenegger “Synthetic gene network restoring endogenous pituitary-thyroid feedback control in experimental Graves' disease“ Proc. Natl. Acad. Sci. U.S.A. 2016, 113:1244. [DOI]
B. Geering, Z. Zokouri, S. Hurlemann, B. Gerrits, D. Auslander, A. Britschgi, M. P. Tschan, H. U. Simon, M. Fussenegger “Identification of Novel Death-Associated Protein Kinase 2 Interaction Partners by Proteomic Screening Coupled with Bimolecular Fluorescence Complementation“ Mol. Cell. Biol. 2016, 36:132-43. [DOI]
L. Lina Schukur, B. Geering, G. Charpin-El Hamri, M. Fussenegger “Implantable synthetic cytokine converter cells with AND-gate logic treat experimental psoriasis“ Sci. Transl. Med. 2015, 7:DOI: 10.1126/scitranslmed.aac4964. [DOI]
B. C. Heng, D. Aubel, M. Fussenegger “Prosthetic gene networks as an alternative to standard pharmacotherapies for metabolic disorders“ Curr. Opin. Biotechnol. 2015, 35:37-45. [DOI]
L. Schukur, B. Geering, M. Fussenegger “Human whole-blood culture system for ex vivo characterization of designer-cell function“ Biotechnol. Bioeng. 2015, 113:588. [DOI]
V. Haellman, M. FusseneggerV. Hällman “Synthetic Biology-Toward Therapeutic Solutions“ J. Mol. Biol. 2015, 428:945. [DOI]
H. Wang, H. Ye, M. Xie, M. Daoud El-Baba, M. Fussenegger “Cosmetics-triggered percutaneous remote control of transgene expression in mice“ Nucleic Acids Res. 2015, 43:e91. [DOI]
R. Kojima, D. Aubel, M. Fussenegger “Novel theranostic agents for next-generation personalized medicine: small molecules, nanoparticles, and engineered mammalian cells“ Curr. Opin. Chem. Biol. 2015, 28:29. [DOI]
M. Xie, M. Fussenegger “Mammalian designer cells: Engineering principles and biomedical applications.“ Biotechnol. J. 2015, 10:1005. [DOI]
T. Kim, M. Folcher, M. Doaud-El Baba, M. Fussenegger “A Synthetic Erectile Optogenetic Stimulator Enabling Blue-Light-Inducible Penile Erection“ Angew. Chem. Int. Ed. 2015, 54:5933. [DOI] [More Information]
T. Kim, M. Folcher, G. Charpin-El Hamri, M. Fussenegger “A synthetic cGMP-sensitive gene switch providing Viagra((R))-controlled gene expression in mammalian cells and mice“ Metab. Eng. 2015, 29:169-79. [DOI]
B. Geering, M. Fussenegger “Synthetic immunology: modulating the human immune system“ Trends Biotechnol. 2015, 33:65-79. [DOI]