Martin Fussenegger - NCCR MSE

Martin Fussenegger

Department of Biosystems Science and Engineering (D-BSSE), ETH Zurich

Work Package Leader WP3: From Cellular Systems to Health Control

Project Leader


C. Yang, F. Sesterhenn, J. Bonet, E. A. van Aalen, L. Scheller, L. A. Abriata, J. T. Cramer, X. Wen, S. Rosset, S. Georgeon, T. Jardetzky, T. Krey, M. Fussenegger, M. Merkx, B. Correia “Bottom-up de novo design of functional proteins with complex structural features“ Nat. Chem. Biol. 2021. [DOI]
F. Teng, M. Fussenegger “Shedding Light on Extracellular Vesicle Biogenesis and Bioengineering“ Adv. Sci. 2020. [DOI]
M. Xie, M. Viviani, M. Fussenegger “Engineering Precision Therapies: Lessons and Motivations from the Clinic“ Synth. Biol. 2020. [DOI]
D. Ausländer, P. F. Lang, M. Kemi, M. Fussenegger “Design of Multipartite Transcription Factors for Multiplexed Logic Genome Integration Control in Mammalian Cells“ ACS Synth. Biol. 2020. [DOI]
M. Mansouri, S. Lichtenstein, T. Strittmatter, P. Buchmann, M. Fussenegger “Construction of a Multiwell Light-Induction Platform for Traceless Control of Gene ExpressionGeneexpression in Mammalian Cells“ Photoswitching Proteins : Methods and Protocols 2020. [DOI]
L. Scheller, M. Schmollack, A. Bertschi, M. Mansouri, P. Saxena, M. Fussenegger “Phosphoregulated orthogonal signal transduction in mammalian cells“ Nat. Commun. 2020. [DOI]
K. Krawczyk, S. Xue, P. Buchmann, G. Charpin-El-Hamri, P. Saxena, M. Hussherr, J. Shao, H. Ye, M. Xie, M. Fussenegger “Electrogenetic cellular insulin release for real-time glycemic control in type 1 diabetic mice“ Science 2020. [DOI]
P. Stücheli, S. Sieber, D. W. Fuchs, L. Scheller, T. Strittmatter, P. Saxena, K. Gademann, M. Fussenegger “Genetically encoded betaxanthin-based small-molecular fluorescent reporter for mammalian cells“ Nucleic Acids Res. 2020. [DOI]
C. E. Meyer, J. Liu, I. Craciun, D. Wu, H. Wang, M. Xie, M. FusseneggerC. G. Palivan “Segregated Nanocompartments Containing Therapeutic Enzymes and Imaging Compounds within DNA-Zipped Polymersome Clusters for Advanced Nanotheranostic Platform“ Small 2020. [DOI]
K. Krawczyk, L. Scheller, H. Kim, M. Fussenegger “Rewiring of endogenous signaling pathways to genomic targets for therapeutic cell reprogramming“ Nat. Commun. 2020. [DOI]
B. M. Gaub, K. C. Kasuba, E. Mace, T. Strittmatter, P. R. Laskowski, S. A. Geissler, A. Hierlemann, M. FusseneggerB. RoskaD. J. Müller “Neurons differentiate magnitude and location of mechanical stimuli“ Proc. Natl. Acad. Sci. U.S.A. 2019. [DOI]
D. Bojar, M. Fussenegger “The Role of Protein Engineering in Biomedical Applications of Mammalian Synthetic Biology“ Small 2019. [DOI]
P. Bai, Y. Liu, S. Xue, G. C. Hamri, P. Saxena, H. Ye, M. Xie, M. Fussenegger “A fully human transgene switch to regulate therapeutic protein production by cooling sensation“ Nat. Med. 2019. [DOI]
H. Chassin, M. Müller, M. Tigges, L. Scheller, M. Lang, M. Fussenegger “A modular degron library for synthetic circuits in mammalian cells“ Nat. Commun. 2019. [DOI]
L. Scheller, M. Fussenegger “From synthetic biology to human therapy: engineered mammalian cells“ Curr. Opin. Biotechnol. 2019, 58:108-16. [DOI]
H. Kim, D. Bojar, M. Fussenegger “A CRISPR/Cas9-based central processing unit to program complex logic computation in human cells“ Proc. Natl. Acad. Sci. U.S.A. 2019. [DOI]
F. Tolle, P. Stücheli, M. Fussenegger “Genetic circuitry for personalized human cell therapy“ Curr. Opin. Biotechnol. 2019, 59:31-38. [DOI]
D. Bojar, T. Fuhrer, M. Fussenegger “Purity by design: Reducing impurities in bioproduction by stimulus-controlled global translational downregulation of non-product proteins“ Metab. Eng. 2018. [DOI]
M. Mansouri, T. Strittmatter, M. Fussenegger “Light-Controlled Mammalian Cells and Their Therapeutic Applications in Synthetic Biology“ Adv. Sci. 2018. [DOI]
Y. Liu, G. Charpin-El Hamri, H. Ye, M. Fussenegger “A synthetic free fatty acid-regulated transgene switch in mammalian cells and mice“ Nucleic Acids Res. 2018. [DOI]
A. P. Teixeira, M. Fussenegger “Engineering mammalian cells for disease diagnosis and treatment“ Curr. Opin. Biotechnol. 2018. [DOI]
D. Bojar, M. Fussenegger “Programming mammalian gene expression with the antibiotic simocyclinone D8 and the flavonoid luteolin“ AIChE J. 2018. [DOI]
Y. Liu, P. Bai, A. Woischnig, G. Charpin-El Hamri, H. Ye, M. Folcher, M. Xie, N. Khanna, M. Fussenegger “Immunomimetic Designer Cells Protect Mice from MRSA Infection“ Cell 2018. [DOI]
D. Bojar, L. Scheller, G. C. Hamri, M. Xie, M. Fussenegger “Caffeine-inducible gene switches controlling experimental diabetes“ Nat. Commun. 2018. [DOI]
M. Xie, M. Fussenegger “Designing cell function: assembly of synthetic gene circuits for cell biology applications“ Nat. Rev. Mol. Cell Biol. 2018. [DOI]
Y. Okamoto, R. Kojima, F. Schwizer, E. Bartolami, T. Heinisch, S. MatileM. FusseneggerT. R. Ward “A cell-penetrating artificial metalloenzyme regulates a gene switch in a designer mammalian cell“ Nat. Commun. 2018. [DOI]
R. Kojima, M. Fussenegger “Engineering Whole Mammalian Cells for Target‐Cell‐Specific Invasion/Fusion“ Adv. Sci. 2018. [DOI]
L. Scheller, T. Strittmatter, D. Fuchs, D. Bojar, M. Fussenegger “Generalized extracellular molecule sensor platform for programming cellular behavior“ Nat. Chem. Biol. 2018. [DOI]
A. Tastanova, M. Folcher, M. Müller, G. Camenisch, A. Ponti, T. Horn, M. S. Tikhomirova, M. Fussenegger “Synthetic biology-based cellular biomedical tattoo for detection of hypercalcemia associated with cancer“ Sci. Transl. Med. 2018. [DOI]
R. Kojima, D. Bojar, G. Rizzi, G. C. Hamri, M. D. El-Baba, P. Saxena, S. Ausländer, K. R. Tan, M. Fussenegger “Designer exosomes produced by implanted cells intracerebrally deliver therapeutic cargo for Parkinson’s disease treatment“ Nat. Commun. 2018. [DOI]
H. Wang, M. Xie, G. Charpin-El Hamri, H. Ye, M. Fussenegger “Treatment of chronic pain by designer cells controlled by spearmint aromatherapy“ Nat. Biomed. Eng. 2018. [DOI]
S. Ausländer, X. Pierrat, L. Hellmann, L. Rachid, M. Fussenegger “Programmable full-adder computations in communicating three-dimensional cell cultures“ Nat. Methods 2017. [DOI]
R. Kojima, L. Scheller, M. Fussenegger “Nonimmune cells equipped with T-cell-receptor-like signaling for cancer cell ablation“ Nat. Chem. Biol. 2017. [DOI]
H. Chassin, B. Geering, L. Schukur, D. Ausländer, B. Lang, M. Fussenegger “Sensing and responding to allergic response cytokines through a genetically encoded circuit“ Nat. Commun. 2017. [DOI]
V. Haellman, M. Fussenegger “Synthetic biology – Engineering cell-based biomedical devices“ Curr. Opin. Biomed. Eng. 2017. [DOI]
F. Sedlmayer, T. Jaeger, U. Jenal, M. Fussenegger “Quorum-Quenching Human Designer Cells for Closed-Loop Control of Pseudomonas aeruginosa Biofilms“ Nano Lett. 2017. [DOI]
P. Saxena, D. Bojar, H. Zulewski, M. Fussenegger “Generation of glucose-sensitive insulin-secreting beta-like cells from human embryonic stem cells by incorporating a synthetic lineage-control network“ J. Biotechnol. 2017. [DOI]
M. Xie, D. Aubel, M. Fussenegger “Closed-loop control systems – The quest for precision therapies for diabetes“ Curr. Opin. Syst. Biol. 2017. [DOI]
A. P. Teixeira, M. Fussenegger “Synthetic biology-inspired therapies for metabolic diseases“ Curr. Opin. Biotechnol. 2017. [DOI]
D. Ausländer, M. Fussenegger “Synthetic Biology‐The Synthesis of Biology“ Angew. Chem. Int. Ed. 2017, 56:6396-419. [DOI]
S. Ausländer, M. Fussenegger “Synthetic RNA-based switches for mammalian gene expression control“ Curr. Opin. Biotechnol. 2017, 48:54-60. [DOI]
M. Muller, D. Auslander, A. Spinnler, J. Sikorski, M. Folcher, M. Fussenegger “Designed cell consortia as fragrance-programmable analog-to-digital converters“ 2017, 13:309-16. [DOI]
M. Xie, H. Ye, H. Wang, G. Charpin-El Hamri, C. Lormeau, P. Saxena, J. StellingM. Fussenegger “β-cell–mimetic designer cells provide closed-loop glycemic control“ Science 2016, 354:1296-1301. [DOI]
R. Kojima, D. Aubel, M. Fussenegger “Toward a world of theranostic medication: Programming biological sentinel systems for therapeutic intervention“ Adv. Drug Delivery Rev. 2016, 105:66-76. [DOI]
M. Xie, V. Haellman, M. FusseneggerV. Hällman “Synthetic biology-application-oriented cell engineering.“ Curr. Opin. Biotechnol. 2016, 40:139. [DOI]
P. Saxena, B. C. Heng, P. Bai, M. Folcher, H. Zulewski, M. Fussenegger “A programmable synthetic lineage-control network that differentiates human IPSCs into glucose-sensitive insulin-secreting beta-like cells“ Nat. Commun. 2016, 7:DOI:10.1038/ncomms11247. [DOI] [More Information]
P. Bai, H. Ye, M. Xie, P. Saxena, H. Zulewski, G. Charpin-El Hamri, V. Djonov, M. Fussenegger “A synthetic biology-based device prevents liver injury in mice“ J. Hepatol. 2016, 65:84. [DOI]
D. Auslaender, D. W. Fuchs, S. Huerlemann, M. Fussenegger “Engineering a ribozyme cleavage-induced split fluorescent aptamer complementation assay“ Nucleic Acids Res. 2016, 44:e94. [DOI]
A. Tastanova, A. Schulz, A. Tolstrup, A. Puklowski, H. Kaufmann, M. Fussenegger “Overexpression of YY1 increases the protein production in mammalian cells“ J. Biotechnol. 2016, 219:72. [DOI]
P. Saxena, G. Charpin-El Hamri, M. Folcher, H. Zulewski, M. Fussenegger “Synthetic gene network restoring endogenous pituitary-thyroid feedback control in experimental Graves' disease“ Proc. Natl. Acad. Sci. U.S.A. 2016, 113:1244. [DOI]
B. Geering, Z. Zokouri, S. Hurlemann, B. Gerrits, D. Auslander, A. Britschgi, M. P. Tschan, H. U. Simon, M. Fussenegger “Identification of Novel Death-Associated Protein Kinase 2 Interaction Partners by Proteomic Screening Coupled with Bimolecular Fluorescence Complementation“ Mol. Cell. Biol. 2016, 36:132-43. [DOI]
L. Lina Schukur, B. Geering, G. Charpin-El Hamri, M. Fussenegger “Implantable synthetic cytokine converter cells with AND-gate logic treat experimental psoriasis“ Sci. Transl. Med. 2015, 7:DOI: 10.1126/scitranslmed.aac4964. [DOI]
B. C. Heng, D. Aubel, M. Fussenegger “Prosthetic gene networks as an alternative to standard pharmacotherapies for metabolic disorders“ Curr. Opin. Biotechnol. 2015, 35:37-45. [DOI]
L. Schukur, B. Geering, M. Fussenegger “Human whole-blood culture system for ex vivo characterization of designer-cell function“ Biotechnol. Bioeng. 2015, 113:588. [DOI]
V. Haellman, M. FusseneggerV. Hällman “Synthetic Biology-Toward Therapeutic Solutions“ J. Mol. Biol. 2015, 428:945. [DOI]
H. Wang, H. Ye, M. Xie, M. Daoud El-Baba, M. Fussenegger “Cosmetics-triggered percutaneous remote control of transgene expression in mice“ Nucleic Acids Res. 2015, 43:e91. [DOI]
R. Kojima, D. Aubel, M. Fussenegger “Novel theranostic agents for next-generation personalized medicine: small molecules, nanoparticles, and engineered mammalian cells“ Curr. Opin. Chem. Biol. 2015, 28:29. [DOI]
M. Xie, M. Fussenegger “Mammalian designer cells: Engineering principles and biomedical applications.“ Biotechnol. J. 2015, 10:1005. [DOI]
T. Kim, M. Folcher, M. Doaud-El Baba, M. Fussenegger “A Synthetic Erectile Optogenetic Stimulator Enabling Blue-Light-Inducible Penile Erection“ Angew. Chem. Int. Ed. 2015, 54:5933. [DOI] [More Information]
T. Kim, M. Folcher, G. Charpin-El Hamri, M. Fussenegger “A synthetic cGMP-sensitive gene switch providing Viagra((R))-controlled gene expression in mammalian cells and mice“ Metab. Eng. 2015, 29:169-79. [DOI]
B. Geering, M. Fussenegger “Synthetic immunology: modulating the human immune system“ Trends Biotechnol. 2015, 33:65-79. [DOI]