Dimitrios Fotiadis - NCCR MSE

Dimitrios Fotiadis

Institute of Biochemistry and Molecular Medicine, University of Bern

Project Leader


N. Ayoub, P. Roth, Z. Ucurum, D. Fotiadis, S. Hirschi “Structural and biochemical insights into His-tag-induced higher-order oligomerization of membrane proteins by cryo-EM and size exclusion chromatography“ J. Struct. Biol. 2023. [DOI]
S. Hirschi, T. R. Ward, W. P. Meier, D. J. MüllerD. Fotiadis “Synthetic Biology: Bottom-Up Assembly of Molecular Systems“ Chem. Rev. 2022. [DOI]
M. Stauffer, J. Jeckelmann, H. Ilgü, Z. Ucurum, R. Boggavarapu, D. Fotiadis “Peptide transporter structure reveals binding and action mechanism of a potent PEPT1 and PEPT2 inhibitor“ Commun. Chem. 2022. [DOI]
F. Ruggeri, C. Schwemmer, M. Stauffer, P. M. Nicollier, J. Figueiredo da Silva, P. D. Bosshart, K. Kochems, D. Fotiadis, A. Knoll, H. Wolf “Placement of Biological Membrane Patches in a Nanofluidic Gap With Control Over Position and Orientation“ 2022. [DOI]
M. Stauffer, Z. Ucurum, D. Harder, D. Fotiadis “Engineering and functional characterization of a proton-driven β-lactam antibiotic translocation module for bionanotechnological applications“ Sci. Rep. 2021. [DOI]
S. Hirschi, D. Kalbermatter, Z. Ucurum, T. Lemmin, D. Fotiadis “Cryo-EM structure and dynamics of the green-light absorbing proteorhodopsin“ Nat. Commun. 2021. [DOI]
M. Stauffer, S. Hirschi, Z. Ucurum, D. Harder, R. Schlesinger, D. Fotiadis “Engineering and Production of the Light-Driven Proton Pump Bacteriorhodopsin in 2D Crystals for Basic Research and Applied Technologies“ Methods Protoc. 2020. [DOI]
S. Hirschi, D. Kalbermatter, Z. Ucurum, D. Fotiadis “Cryo-electron microscopic and X-ray crystallographic analysis of the light-driven proton pump proteorhodopsin reveals a pentameric assembly“ J. Struct. Biol. X 2020. [DOI]
S. Hirschi, D. Fotiadis “Purification of Membrane Proteins by Affinity Chromatography with On-Column Protease Cleavage“ Expression, Purification, and Structural Biology of Membrane Proteins 2020. [DOI]
J. Gaitzsch, S. Hirschi, S. Freimann, D. Fotiadis, W. Meier “Directed Insertion of Light-Activated Proteorhodopsin into Asymmetric Polymersomes from an ABC Block Copolymer“ Nano Lett. 2019. [DOI]
S. Hirschi, N. Fischer, D. Kalbermatter, P. R. Laskowski, Z. Ucurum, D. J. MüllerD. Fotiadis “Design and assembly of a chemically switchable and fluorescently traceable light-driven proton pump system for bionanotechnological applications“ Sci. Rep. 2019. [DOI]
D. Kalbermatter, N. Shrestha, N. Ader-Ebert, M. Herren, P. Moll, R. K. Plemper, K. Altmann, J. P. Langedijk, F. Gall, U. Lindenmann, R. Riedl, D. Fotiadis, P. Plattet “Primary Resistance Mechanism of the Canine Distemper Virus Fusion Protein against a Small-Molecule Membrane Fusion Inhibitor“ Virus Res. 2018. [DOI]
R. Goers, J. Thoma, N. Ritzmann, A. Di Silvestro, C. Alter, G. Gunkel-Grabole, D. FotiadisD. J. Müller, W. Meier “Optimized reconstitution of membrane proteins into synthetic membranes“ Commun. Chem. 2018. [DOI]
J. Thoma, S. Manioglu, D. Kalbermatter, P. D. Bosshart, D. FotiadisD. J. Müller “Protein-enriched outer membrane vesicles as a native platform for outer membrane protein studies“ Commun. Biol. 2018. [DOI]
P. R. Laskowski, M. Pfreundschuh, M. Stauffer, Z. Ucurum, D. FotiadisD. J. Müller “High-Resolution Imaging and Multiparametric Characterization of Native Membranes by Combining Confocal Microscopy and Atomic Force Microscopy-Based Multifunctional Toolbox“ ACS Nano 2017. [DOI]
W. Aeschimann, S. Staats, S. Kammer, N. Olieric, J. Jeckelmann, D. Fotiadis, T. Netscher, G. Rimbach, M. Cascella, A. Stocker “Self-assembled α-Tocopherol Transfer Protein Nanoparticles Promote Vitamin E Delivery Across an Endothelial Barrier“ Sci. Rep. 2017. [DOI]
N. Ritzmann, J. Thoma, S. Hirschi, D. Kalbermatter, D. FotiadisD. J. Müller “Fusion Domains Guide the Oriented Insertion of Light-Driven Proton Pumps into Liposomes“ Biophys J. 2017. [DOI]
D. Kalbermatter, P. Chiu, J. Jeckelmann, Z. Ucurum, T. Walz, D. Fotiadis “Electron crystallography reveals that substrate release from the PTS IIC glucose transporter is coupled to a subtle conformational change“ J. Struct. Biol. 2017. [DOI]
P. Orekhov, A. Bothe, H. Steinhoff, K. V. Shaitan, S. Raunser, D. Fotiadis, R. Schlesinger, J. P. Klare, M. Engelhard “Sensory Rhodopsin I and Sensory Rhodopsin II Form Trimers of Dimers in Complex with their Cognate Transducers“ Photochem. Photobiol. 2017. [DOI]
M. Pfreundschuh, D. Harder, Z. Ucurum, D. FotiadisD. J. Müller “Detecting Ligand-Binding Events and Free Energy Landscape while Imaging Membrane Receptors at Subnanometer Resolution“ Nano Lett. 2017, 17(5):3261-69. [DOI]
D. Harder, S. Hirschi, Z. Ucurum, R. Goers, W. Meier, D. J. MüllerD. Fotiadis “Engineering a Chemical Switch into the Light-driven Proton Pump Proteorhodopsin by Cysteine Mutagenesis and Thiol Modification“ Angew. Chem. Int. Ed.  2016, 55:8846. [DOI]
S. HirschiM. Stauffer, D. Harder, D. J. Müller, W. Meier, D. Fotiadis “Engineering and Assembly of Protein Modules into Functional Molecular Systems“ Chimia 2016, 6:398. [DOI]
R. Boggavarapu, S. Hirschi, D. Harder, M. Meury, Z. Ucurum, M. J. Bergeron, D. Fotiadis “Purification of Human and Mammalian Membrane Proteins Expressed in Xenopus laevis Frog Oocytes for Structural Studies“ Heterologous Expression of Membrane Proteins 2016, 1432:223-42. [DOI]
D. Kalbermatter, J. Jeckelmann, P. Chiu, Z. Ucurum, T. Walz, D. Fotiadis “2D and 3D crystallization of the wild-type IIC domain of the glucose PTS transporter from Escherichia coli“ J. Struct. Biol. 2015, 191:376-80. [DOI]
R. Boggavarapu, J. Jeckelmann, D. Harder, Z. Ucurum, D. Fotiadis “Role of electrostatic interactions for ligand recognition and specificity of peptide transporters“ BMC Biol. 2015, 13:DOI: 10.1186/s12915-015-0167-8. [DOI] [More Information]
D. Fotiadis, A. Engel “Two‐Dimensional Crystallisation of Membrane Proteins and Structural Assessment“ eLS 2015:1-10. [DOI]
R. Petrosyan, C. A. Bippes, S. Walheim, D. Harder, D. Fotiadis, T. Schimmel, D. Alsteens, D. J. Müller “Single-Molecule Force Spectroscopy of Membrane Proteins from Membranes Freely Spanning Across Nanoscopic Pores“ Nano Lett. 2015, 15:3624. [DOI]
P. D. Bosshart, A. Engel, D. Fotiadis “High-Resolution Atomic Force Microscopy Imaging of Rhodopsin in Rod Outer Segment Disk Membranes“ Rhodopsin 2015:189-203. [DOI]