Dimitrios Fotiadis - NCCR MSE

Dimitrios Fotiadis

Institute of Biochemistry and Molecular Medicine, University of Bern

Project Leader


M. Stauffer, Z. Ucurum, D. Harder, D. Fotiadis “Engineering and functional characterization of a proton-driven β-lactam antibiotic translocation module for bionanotechnological applications“ Sci. Rep. 2021. [DOI]
S. Hirschi, D. Kalbermatter, Z. Ucurum, T. Lemmin, D. Fotiadis “Cryo-EM structure and dynamics of the green-light absorbing proteorhodopsin“ Nat. Commun. 2021. [DOI]
M. Stauffer, S. Hirschi, Z. Ucurum, D. Harder, R. Schlesinger, D. Fotiadis “Engineering and Production of the Light-Driven Proton Pump Bacteriorhodopsin in 2D Crystals for Basic Research and Applied Technologies“ Methods Protoc. 2020. [DOI]
S. Hirschi, D. Kalbermatter, Z. Ucurum, D. Fotiadis “Cryo-electron microscopic and X-ray crystallographic analysis of the light-driven proton pump proteorhodopsin reveals a pentameric assembly“ J. Struct. Biol. X 2020. [DOI]
S. Hirschi, D. Fotiadis “Purification of Membrane Proteins by Affinity Chromatography with On-Column Protease Cleavage“ Expression, Purification, and Structural Biology of Membrane Proteins 2020. [DOI]
J. Gaitzsch, S. Hirschi, S. Freimann, D. Fotiadis, W. Meier “Directed Insertion of Light-Activated Proteorhodopsin into Asymmetric Polymersomes from an ABC Block Copolymer“ Nano Lett. 2019. [DOI]
S. Hirschi, N. Fischer, D. Kalbermatter, P. R. Laskowski, Z. Ucurum, D. J. MüllerD. Fotiadis “Design and assembly of a chemically switchable and fluorescently traceable light-driven proton pump system for bionanotechnological applications“ Sci. Rep. 2019. [DOI]
D. Kalbermatter, N. Shrestha, N. Ader-Ebert, M. Herren, P. Moll, R. K. Plemper, K. Altmann, J. P. Langedijk, F. Gall, U. Lindenmann, R. Riedl, D. Fotiadis, P. Plattet “Primary Resistance Mechanism of the Canine Distemper Virus Fusion Protein against a Small-Molecule Membrane Fusion Inhibitor“ Virus Res. 2018. [DOI]
R. Goers, J. Thoma, N. Ritzmann, A. Di Silvestro, C. Alter, G. Gunkel-Grabole, D. FotiadisD. J. Müller, W. Meier “Optimized reconstitution of membrane proteins into synthetic membranes“ Commun. Chem. 2018. [DOI]
J. Thoma, S. Manioglu, D. Kalbermatter, P. D. Bosshart, D. FotiadisD. J. Müller “Protein-enriched outer membrane vesicles as a native platform for outer membrane protein studies“ Commun. Biol. 2018. [DOI]
P. R. Laskowski, M. Pfreundschuh, M. Stauffer, Z. Ucurum, D. FotiadisD. J. Müller “High-Resolution Imaging and Multiparametric Characterization of Native Membranes by Combining Confocal Microscopy and Atomic Force Microscopy-Based Multifunctional Toolbox“ ACS Nano 2017. [DOI]
W. Aeschimann, S. Staats, S. Kammer, N. Olieric, J. Jeckelmann, D. Fotiadis, T. Netscher, G. Rimbach, M. Cascella, A. Stocker “Self-assembled α-Tocopherol Transfer Protein Nanoparticles Promote Vitamin E Delivery Across an Endothelial Barrier“ Sci. Rep. 2017. [DOI]
N. Ritzmann, J. Thoma, S. Hirschi, D. Kalbermatter, D. FotiadisD. J. Müller “Fusion Domains Guide the Oriented Insertion of Light-Driven Proton Pumps into Liposomes“ Biophys J. 2017. [DOI]
D. Kalbermatter, P. Chiu, J. Jeckelmann, Z. Ucurum, T. Walz, D. Fotiadis “Electron crystallography reveals that substrate release from the PTS IIC glucose transporter is coupled to a subtle conformational change“ J. Struct. Biol. 2017. [DOI]
P. Orekhov, A. Bothe, H. Steinhoff, K. V. Shaitan, S. Raunser, D. Fotiadis, R. Schlesinger, J. P. Klare, M. Engelhard “Sensory Rhodopsin I and Sensory Rhodopsin II Form Trimers of Dimers in Complex with their Cognate Transducers“ Photochem. Photobiol. 2017. [DOI]
M. Pfreundschuh, D. Harder, Z. Ucurum, D. FotiadisD. J. Müller “Detecting Ligand-Binding Events and Free Energy Landscape while Imaging Membrane Receptors at Subnanometer Resolution“ Nano Lett. 2017, 17(5):3261-69. [DOI]
D. Harder, S. Hirschi, Z. Ucurum, R. Goers, W. Meier, D. J. MüllerD. Fotiadis “Engineering a Chemical Switch into the Light-driven Proton Pump Proteorhodopsin by Cysteine Mutagenesis and Thiol Modification“ Angew. Chem. Int. Ed.  2016, 55:8846. [DOI]
S. HirschiM. Stauffer, D. Harder, D. J. Müller, W. Meier, D. Fotiadis “Engineering and Assembly of Protein Modules into Functional Molecular Systems“ Chimia 2016, 6:398. [DOI]
R. Boggavarapu, S. Hirschi, D. Harder, M. Meury, Z. Ucurum, M. J. Bergeron, D. Fotiadis “Purification of Human and Mammalian Membrane Proteins Expressed in Xenopus laevis Frog Oocytes for Structural Studies“ Heterologous Expression of Membrane Proteins 2016, 1432:223-42. [DOI]
D. Kalbermatter, J. Jeckelmann, P. Chiu, Z. Ucurum, T. Walz, D. Fotiadis “2D and 3D crystallization of the wild-type IIC domain of the glucose PTS transporter from Escherichia coli“ J. Struct. Biol. 2015, 191:376-80. [DOI]
R. Boggavarapu, J. Jeckelmann, D. Harder, Z. Ucurum, D. Fotiadis “Role of electrostatic interactions for ligand recognition and specificity of peptide transporters“ BMC Biol. 2015, 13:DOI: 10.1186/s12915-015-0167-8. [DOI] [More Information]
D. Fotiadis, A. Engel “Two‐Dimensional Crystallisation of Membrane Proteins and Structural Assessment“ eLS 2015:1-10. [DOI]
R. Petrosyan, C. A. Bippes, S. Walheim, D. Harder, D. Fotiadis, T. Schimmel, D. Alsteens, D. J. Müller “Single-Molecule Force Spectroscopy of Membrane Proteins from Membranes Freely Spanning Across Nanoscopic Pores“ Nano Lett. 2015, 15:3624. [DOI]
P. D. Bosshart, A. Engel, D. Fotiadis “High-Resolution Atomic Force Microscopy Imaging of Rhodopsin in Rod Outer Segment Disk Membranes“ Rhodopsin 2015:189-203. [DOI]