- NCCR MSE

Conformational Thermo-stabilization of Functional Biomolecular Modules and development of light-con-trolled Molecular Switches

Simplified scheme of GPCR signalling: Light or ligand binding triggers a conformational change of the receptor that induces G protein activation.
Simplified scheme of GPCR signalling: Light or ligand binding triggers a conformational change of the receptor that induces G protein activation.
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Publications

N. Romantini, S. Alam, S. Dobitz, M. Spillmann, M. De Foresta, R. Schibli, G. F. X. Schertler, H. Wennemers, X. Deupi, M. Behe, P. Berger “Exploring the signaling space of a GPCR using bivalent ligands with a rigid oligoproline backbone“ Proc. Natl. Acad. Sci. U.S.A. 2021. [DOI]
M. Spillmann, L. Thurner, N. Romantini, M. Zimmermann, B. Meger, M. Behe, M. Waldhoer, G. F. X. Schertler, P. Berger “New Insights into Arrestin Recruitment to GPCRs“ Int. J. Mol. Sci. 2020. [DOI]
T. Weinert, V. Panneels “Membrane Protein Preparation for Serial Crystallography Using High-Viscosity Injectors: Rhodopsin as an Example“ Expression, Purification, and Structural Biology of Membrane Proteins 2020. [DOI]
N. Varma, E. Mutt, J. Mühle, V. Panneels, A. Terakita, X. Deupi, P. Nogly, G. F. X. Schertler, E. Lesca “Crystal structure of jumping spider rhodopsin-1 as a light sensitive GPCR“ Proc. Natl. Acad. Sci. U.S.A. 2019, 116(29):14547-56. [DOI]
D. Ehrenberg, N. Varma, X. Deupi, M. Koyanagi, A. Terakita, G. F. X. Schertler, J. Heberle, E. Lesca “The two-photon reversible reaction of the bistable jumping spider rhodopsin-1“ Biophys. J. 2019. [DOI]
M. Hilbert, A. Noga, D. Frey, V. Hamel, P. Guichard, S. H. Kraatz, M. Pfreundschuh, S. Hosner, I. Flückiger, R. Jaussi, M. M. Wieser, K. M. Thieltges, X. Deupi, D. J. Mülle, R. Kammerer, P. Gönczy, M. Hirono, M. O. Steinmetz, D. J. Müller “SAS-6 engineering reveals interdependence between cartwheel and microtubules in determining centriole architecture“ Nat. Cell Biol. 2016, 18:393. [DOI]
D. Sun, T. Flock, X. Deupi, S. Maeda, M. Matkovic, S. Mendieta, D. Mayer, R. J. Dawson, G. Schertler, M. M. Babu, D. B. Veprintsev “Probing Gαi1 protein activation at single–amino acid resolution“ Nat. Struct. Mol. Biol. 2015, 22:686. [DOI]
F. M. Heydenreich, Z. Vuckovic, M. Matkovic, D. B. Veprintsev “Stabilization of G protein-coupled receptors by point mutations“ Front. Pharmacol. 2015, 6:82. [DOI] [More Information]
D. Milić, D. B. Veprintsev “Large-scale production and protein engineering of G protein-coupled receptors for structural studies“ Front. Pharmacol. 2015, 6:66. [DOI] [More Information]
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